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Figure 5: 𝛼 -ALCAM-conjugated PLNs show cell-specific targeting in a time-dependent manner.   𝛼 -ALCAM-conjugated PLNs were incubated with osteosarcoma cells for 0, 1, 2, and 4 hours then washed off.   𝛼 -ALCAM targeted PLNs bind specifically to the ALCAM expressing osteosarcoma cell line KHOS240s at one hour and reach a maximum binding at 4 hours of incubation. Fluorescence microscopy of PLNs (shown in red) with DAPI (blue) nuclear counterstaining (magnification 20x).