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Figure 6: 𝛼 -ALCAM-conjugated PLNs are internalized via receptor-mediated endocytosis.   𝛼 -ALCAM-targeted PLNs incubated with the osteosarcoma cell line KHOS240s at 4 degrees and at 37 degrees Celsius show inhibition of receptor-mediated endocytosis at 4°C and rapid internalization of targeted PLNs at 37°C versus untargeted PLNs that do not bind under either circumstance. Fluorescence microscopy of PLNs (shown in red) with DAPI (blue) nuclear counterstaining (magnification 20x).