Characterization of Liposarcoma Cell Lines for Preclinical and Biological Studies

Figure 6

Heat map summarizing the LPS functional analyses and marker expression. Black indicates aggressive signature, grey indicates moderately aggressive signature, and white indicates a nonaggressive signature. Cell lines considered highly aggressive displayed a high proliferation rate; ability to form tumor; resistance towards differentiation; high capacity for colony formation, migration and invasion; low expression of differentiation markers (CEBPB, CEBPA, PPARG, FABP4); high expression of pluripotency markers NANOG, OCT4, SOX2; high expression of stem cell surface markers CD90, CD105, CD73, and CD44; expression of cancer stem cell marker CD133 and Aldefluor in more than 3% of the cells. Cell lines considered moderately aggressive displayed the ability to undergo spontaneous differentiation; moderate capacity to form colonies and to migrate or invade; moderate expression of differentiation markers (CEBPB, CEBPA, PPARG, FABP4) pluripotency markers NANOG, OCT4, SOX2, and stem cell surface markers CD90, CD105, CD73, and CD44; CD133 and Aldefluor expression in (0.1–3%) of the cells. Cell lines considered non-aggressive displayed a low proliferation rate, did not form tumors in  vivo; adipocytic differentiated at basal level; low capacity for colony formation, migration and invasion; high expression of differentiation markers CEBPB, CEBPA, PPARG, FABP4; low expression of pluripotency markers NANOG, OCT4, SOX2; expression of stem cell surface markers CD90, CD105, CD73, and CD44 in a low percentage of the cells and undetectable expression of the cancer stem cell markers.