Figure 3: Migration assay. (a) A single representative wound healing assay (10x magnification), which showed no significant differences, , between the variants and the parental cell line MSC-TS at (A) 0 hour, (B) 12 hours, (C) 24 hours, and (D) 48 hours. (E) Quantitative measurement of wound gap by Image J software also showed no statistical significance between clones and the parental line. (b) A summary of the Boyden chamber experiment results; BSA coated wells serve as control along with HOS cells. Bars, standard deviation (SD). Experiments were replicated three times.