Proteomic Technologies for the Study of Osteosarcoma

Figure 2

LC MS/MS workflow. Proteins can be separated by 2D or SDS-PAGE prior to mass spectrometry. Proteins are digested into peptides, separated by LC, and identified by mass spectrometry. The mass spectra are searched in a protein database to identify proteins. The proteins are quantified using spectral counting (𝑘 is a given protein, SpC is the spectral count, 𝐿 is the length of the protein, and 𝑁 is all proteins identified in the gel lane) or peak areas in label-free proteomics.