Sarcoma Stem Cells: Do We Know What We Are Looking for?

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr class="thead"><td align="left">Technique</td><td align="left">Description</td></tr><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr><td align="left">Surface markers (e.g., CD133)</td><td align="left">Cells are incubated with fluorescent antibodies to specific surface markers. Flow-cytometry is used to isolate cells expressing surface marker</td></tr><tr><td align="center" colspan="2"><hr/></td></tr><tr><td align="left">Aldehyde Dehydrogenase (ALDH)</td><td align="left">Cells are incubated with reagent that is activated to fluorescent state by ALDH. Flow-cytometry is used to isolate the cells with the most ALDH activity </td></tr><tr><td align="center" colspan="2"><hr/></td></tr><tr><td align="left">Side population analysis (dye exclusion)</td><td align="left">Cells are incubated with a fluorescent dye such as Hoechst 33342. Flow-cytometry is used to select the population that excludes the dye, referred to as the “side population,” because of its location on the left-most portion of the flowplot</td></tr><tr class="table-tr"><td colspan="2"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Techniques used to isolate sarcoma-initiating cells.

Sarcoma

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Table 1

Table 1: Sarcoma Stem Cells: Do We Know What We Are Looking for? 