| Tumor Type | Technique | Summary of findings | Reference |
| Osteosarcoma | | CD133+ cells demonstrated increased sphere formation, growth in soft agar, expression of OCT3/4, and presence of side population | | CD133 | [21, 22] | | | | Double positive (CD117+ and Stro-1+) cells were seen with a higher incidence in spheres, and they showed higher tumorigenicity as well as chemoresistance | | CD117, Stro-1 | [23] | | | | ALDH-high cells isolated from xenografts established from cell line OS99-1 had increased tumorigenicity, self-renewal, and ability to produce differentiated progeny, and expressed increased levels of OCT3/4A, NANOG, and SOX-2 | | | | ALDH | [24] | | | | | | SP was seen in 1 of 7 osteosarcoma cell lines tested. SP population had increased sphere- and colony-forming activity and increased tumorigenicity | | SP | [25] | | |
| Ewing’s sarcoma | | CD133+ cells showed increased tumorigenicity, ability to establish a heterogeneous population and differentiation, and increased expression of OCT4, SOX2, and NANOG. There was a correlation in primary tumors between higher expression of CD133 and chemoresistance | | | | CD133 | [26–28] | | | | | | ALDH-high cells showed increased sphere- and colony-forming ability, chemoresistance, SP, and tumorigenicity | | ALDH | [35] | | |
| Rhabdomyosarcoma | | Serial passages of rhabdomyosarcoma spheres enriched for cells with increased expression of OCT4, NANOG, c-Myc, SOX2, and PAX3 and increased expression of CD133 and CD133+cells showed increased tumorigenicity, ability to Differentiate, and chemoresistance. CD133 expression also correlated with poorer survival in patient samples | | | | | | CD133 | [30] | | | | | | |
| Synovial sarcoma | | 5 of 5 primary tumor samples and 3 of 3 cell lines demonstrate a CD133+ subpopulation, but not TIC properties were tested | | CD133 | [31] | | |
| Multiple sarcomas | | Size of SP in primary tumor samples correlated with tumor grade. SP cells from 1 osteosarcoma, 1 synovial sarcoma, and 2 malignant fibrous histiocytosis samples showed increased tumorigenicity and ability to produce a heterogeneous cell population (SP and nonSP) | | | | SP | [32] | | | | |
|
|