Research Article
rAAV Vectors as Safe and Efficient Tools for the Stable Delivery of Genes to Primary Human Chondrosarcoma Cells In Vitro and In Situ
Table 2
Histomorphometric analyses in transduced human chondrosarcoma cells in situ (day 20).
| | Assay | No vector | rAAV-lacZ |
| | Cell densities (cells/mm2) | 394 (6) | 401 (8) | | Ki-67 staining (%) | 52 (3) | 54 (4) | | TUNEL staining (%) | 2 (1) | 1 (1) | | Matrix staining (%) | 85 (2) | 83 (3) | | Type II collagen staining (pixels) | 79 (3) | 80 (4) | | Type I collagen staining (pixels) | 8 (2) | 10 (3) | | Type X collagen staining (%) | 79 (3) | 77 (4) | | SOX9 staining (%) | 84 (2) | 83 (3) | | S-100 staining (%) | 82 (3) | 81 (2) |
|
|
Values are given as mean of all cultures (SD).
|
