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Figure 3: To identify proteins associated with the prognosis and malignant grade of osteosarcoma, we conducted a 2D-DIGE study using osteosarcoma biopsy samples. A hierarchical cluster analysis of 17 osteosarcoma showed that there were 72 protein spots that had different intensity out of a total of 1457 protein spots ( ). The expected value of this study (from 1457 total protein spots and ) was >73 protein spots. Therefore, the study design also could not obtain a sufficient number of protein spots which had statistically significant differences.