Discovery of Biomarkers for Osteosarcoma by Proteomics Approaches

Table 2

Summary of the platforms in proteomic analysis.

	Gel-based methods		Gel free methods		Microarray
	2D gel electrophoresis	2D-DIGE	SELDI and MALDI	LC-MS	Protein array
Advantage	Separation of large number of proteins	Reliable quantification; Required small amount of proteins; Reproducible; Separation of large number of proteins	Automation; Required small amount of proteins; Possibility of quantification	Automation; Required small amount of proteins; Possibility of quantification	High-throughput; Automation; High reproducible; Required small amount of proteins; Sensitive detection for post translational modification
Disadvantage	Required large amount of proteins; Nonautomation; Limitation for large and small proteins in directions	Nonautomaition; Limitation for large and small proteins in directions	Less reliable in protein direction; Less detection of low abundance proteins	Less detection of low abundance proteins	Limitation for the total number of proteins

2D-DIGE: 2-dimensional fluorescence difference gel electrophoresis, SELDI: surface-enhanced laser desorption/ionization, MALDI: matrix-assisted laser desorption/ionization, LC-MS: liquid chromatography mass spectrometry.