Table 2: Summary of the platforms in proteomic analysis.

Gel-based methodsGel free methodsMicroarray
2D gel electrophoresis2D-DIGESELDI and MALDILC-MSProtein array

AdvantageSeparation of large number of proteinsReliable quantification
Required small amount of proteins
Reproducible
Separation of large number of proteins
Automation
Required small amount of proteins
Possibility of quantification
Automation
Required small amount of proteins
Possibility of quantification
High-throughput
Automation
High reproducible
Required small amount of proteins
Sensitive detection for post translational modification

DisadvantageRequired large amount of proteins
Nonautomation
Limitation for large and small proteins in directions
Nonautomaition
Limitation for large and small proteins in directions
Less reliable in protein direction
Less detection of low abundance proteins
Less detection of low abundance proteinsLimitation for the total number of proteins

2D-DIGE: 2-dimensional fluorescence difference gel electrophoresis, SELDI: surface-enhanced laser desorption/ionization, MALDI: matrix-assisted laser desorption/ionization, LC-MS: liquid chromatography mass spectrometry.