Osteosarcoma Phenotype Is Inhibited by 3,4-Methylenedioxy-β-nitrostyrene

Figure 4

MNS disrupts preformed 143B colonies. Images of the metastatic 143B cell colonies incubated for 24 hours in presence of 10 μM MNS, 10 μM of the inactive analogue, or 1% DMSO as a vehicle control. The membrane-impermanent fluorescent stain 4′, 6-diamidino-2-phenylindole (DAPI, 0.1 μg/mL) was added 8 hours after incubation began. Fluorescent microscopy was used to obtain photomicrographs (bottom panel). This figure shows representative images from three independent experiments, each with two to three wells per group.