479712.fig.009a
(a)
479712.fig.009b
(b)
Figure 9: MNS does not inhibit activity of any of the 16 tyrosine kinases that were tested, including syk and src. In panel (a), the effect of 10 μM MNS was examined in the presence of the concentration of ATP that approximates the Km for the individual kinase. In panel (b), the effect of 10 or 100 μM MNS was examined in the presence of 10 μM ATP or the concentration of ATP that approximates the Km for src or syk. All assays were performed in either triplicate or quadruplicate.