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Sarcoma
Volume 2012 (2012), Article ID 620834, 12 pages
http://dx.doi.org/10.1155/2012/620834
Research Article

Conditional Inactivation of Pten with EGFR Overexpression in Schwann Cells Models Sporadic MPNST

1Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
2Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA
4Brain Tumor Program, University of Minnesota, Minneapolis, MN 55455, USA
5Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
6Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32610, USA
7Division of Experimental Hematology and Cancer Biology, Cincinnati Children’s Hospital Research Foundation, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 45229, USA
8Division of Pathology and Laboratory Medicine, Cincinnati Children’s Hospital Research Foundation, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 45229, USA
9UF Shands Cancer Center, Genetics Institute, University of Florida, Gainesville, FL 32610, USA
10Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA

Received 6 August 2012; Accepted 2 November 2012

Academic Editor: R. Pollock

Copyright © 2012 Vincent W. Keng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: shows the histological and immunohistochemical analyses of peripheral nervous system phenotype in Dhh-Cre; Ptenflox/flox (ΔPten) animals. The relatively low numbers of Ki67-positive cells detectable in peripheral nervous tissue sections of ΔPten animals indicate a low-grade peripheral nerve sheath tumor (PNST) phenotype. As expected, pAkt levels were much higher in the peripheral nervous tissue sections of ΔPten animals when compared with wild-type FVB/N (FVB) control animals. pErk levels in all peripheral nervous tissue sections were comparable between ΔPten and FVB animals. All peripheral nervous tissue sections taken from ΔPten and FVB animals were positive for Olig2 staining, consistent with nerve association.

Supplementary Figure 2: demonstrates the high-grade PNSTs that develop in our mouse model recapitulate human sporadic malignant peripheral nerve sheath tumors (MPNSTs). Using high power view of hematoxylin-eosin (HE) stained PNSTs, key phenotypic features seen in human MPNSTs were also present in tumors taken from our mouse model. These include hypercellularity, haphazard cell arrangement, poor cell differentiation, nuclear pleomorphism and nuclear hyperchromasia. In addition, PNSTs taken from our mouse model were highly reactive for Ki67, indicating high mitotic activity, similar to human high-grade tumors.

  1. Supplementary Figure 1
  2. Supplementary Figure 2