782970.fig.006a
(a)
782970.fig.006b
(b)
782970.fig.006c
(c)
782970.fig.006d
(d)
Figure 6: IGF1 increases de novo Cer formation. Levels of (a) C16 : 0-Cer and (b) C18 : 0-, C22 : 0-, C24 : 0-, and C24 : 1-Cer in VH-64 cells incubated for the indicated times with IGF1 (100 ng/mL). (c) Levels of sphinganine (SA) and sphingosine (SO) in VH-64 cells treated for 72 hours with IGF1 (100 ng/mL) in the absence and presence of FB1 (30 μM). (d) Percentage of different Cer species in VH-64 cells treated for 72 hours with IGF1 (100 ng/mL) in the absence (white columns) and presence (grey columns) of FB1 (30 μM). Values of vehicle-treated controls for the different Cer species were set to 100%. Error bars: mean ± SD of triplicate determinations.