782970.fig.007a
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782970.fig.007b
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782970.fig.007c
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Figure 7: Functional consequences of Cer formation. (a) Percentage of viable VH-64 cells treated for 72 hours without ( , ●) and with (□, ■) IGF1 (100 ng/mL) in the absence (open symbols) and presence (closed symbols) of FB1 (30 μM), followed by incubation for 18 hours with the indicated concentrations of Apo2L/TRAIL. (b) EIA analysis of XIAP in VH-64 cells treated for 72 hours without and with IGF1 (100 ng/mL), in the presence of the indicated concentrations of FB1. (c) Ratio of phosphorylated AKT to total AKT determined by ELISA in VH-64 cells treated for 72 hours without and with IGF1 (100 ng/mL) in presence of the indicated concentrations of FB1. Asterisks: * , ** ; error bars: mean ± SD of quadruplicate determinations from 3 independent experiments.