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Sarcoma
Volume 2013 (2013), Article ID 365723, 12 pages
http://dx.doi.org/10.1155/2013/365723
Research Article

Rapid Screening of Novel Agents for Combination Therapy in Sarcomas

1Chemical Biology and Molecular Medicine, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA
2Translational Research Lab, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA
3Small Animal Imaging Lab, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA
4Sarcoma Program, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA
5Anatomic Pathology Department, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA

Received 6 June 2013; Revised 4 September 2013; Accepted 5 September 2013

Academic Editor: Chandrajit Premanand Raut

Copyright © 2013 Christopher L. Cubitt et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Figure 1S: Mean excess over highest single agent (EOHSA) vs. P value for tested drug combinations in 10 sarcoma cell lines. EOHSA was calculated from cell viability assay dose-response data for drug combinations and individual drugs for each combination. Results from combinations with potentially more significant synergy will show up in the upper right quadrant. Drug combinations are listed in legend in order of the mean EOHSA value with higher values appearing first. Symbol size represents the relative number of experiments included in the mean EOHSA value.

Supplemental Table 1S: Concurrent 72 hour drug combination effect summary. The sarcoma cell line and molar drug ratio for each drug combination is indicated. Combination index (CI) values for effect levels of 0.75, 0.9 and 0.95 were calculated for each independent experiment by the method of Chou and Talalay and the mean CI value “CI(mean)” was calculated using the CI for each effect level. The standard error of the mean for CI values across independent experiments are shown “CI(SEM)”. EoHSA values and level of significance are represented in EoHSA (mean) and –log P-Value columns, respectively. Each line represents the calculated mean value for the number of independent experiments (n).

Supplemental Table 2S: Sequential drug combination effect summary. The sarcoma cell line and molar drug ratio for each drug combination is indicated. Time1 indicates first drug incubation time (hours) for first drug listed in combination. Time2 indicates incubation time (hours) for secondary drug(s) added. Combination index (CI) values for effect levels of 0.75, 0.9 and 0.95 were calculated for each independent experiment by the method of Chou and Talalay and the mean CI value “CI(mean)” was calculated using the CI for each effect level. The standard error of the mean for CI values across independent experiments are shown “CI(SEM)”. EoHSA values and level of significance are represented in EoHSA(mean) and –log P-Value columns, respectively. Each line represents the calculated mean value for the number of independent experiments (n).

  1. Supplementary Materials