Reduced Latency in the Metastatic Niche Contributes to the More Aggressive Phenotype of LM8 Compared to Dunn Osteosarcoma Cells

Table 3

Regulation of potential dormancy genes in LM8 compared to Dunn cells.

	Symbol	Fold change	P value
Genes downregulated in LM8
Basic helix-loop-helix domain containing, class B9	Bhlhb9	−60.98	8.46𝐸−13
Fibronectin 1	Fn1	−2.95	5.69𝐸−08
Transforming growth factor, beta 2	Tgfb2	−2.43	6.09𝐸−06
Rous sarcoma oncogene	Src	−2.36	2.24𝐸−06
Tropomyosin 1, alpha	Tpm1	−2.28	2.53𝐸−07
Transforming growth factor, beta receptor I	Tgfbr1	−2.15	5.06𝐸−06
Genes upregulated in LM8
Connective tissue growth factor	Ctgf	2.72	2.18𝐸−04
Genes not differentially regulated in LM8
Secreted phosphoprotein 1	Spp1	−1.78	2.08𝐸−05
A kinase (PRKA) anchor protein (gravin) 12; SSeCKS	Akap12	−1.67	5.18𝐸−05
plasminogen activator, urokinase receptor; uPAR	Plaur	−1.59	3.80𝐸−04
Cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4); INK4b; p15	Cdkn2b	−1.47	4.66𝐸−03
Discoidin domain receptor family, member 2	Ddr2	−1.46	2.14𝐸−03
Angiomotin	Amot	−1.16	8.07𝐸−02
Thrombospondin 1	Thbs1	−1.13	1.48𝐸−01
Nuclear receptor subfamily 2, group F, member 2	Nr2f2	−1.10	2.62𝐸−01
Basic helix-loop-helix family, member e41; Sharp1; Bhlhb3; Bhlhb2l	Bhlhe41	−1.09	2.22𝐸−01
Smad family member 7	Smad7	−1.07	5.07𝐸−01
NME/NM23 nucleoside diphosphate kinase 1	Nme1	−1.03	6.95𝐸−01
Insulin-like growth factor binding protein 5	IGFBP5	−1.03	7.56𝐸−01
Integrin alpha 5 (fibronectin receptor alpha)	Itga5	−1.02	8.71𝐸−01
Mitogen-activated protein kinase kinase 7; MKK7	Map2k7	−1.01	9.48𝐸−01
Rho, GDP dissociation inhibitor (GDI) beta; RhoGD12	Arhgdib	−1.01	9.05𝐸−01
Mitogen-activated protein kinase 14; p38	Mapk14	−1.01	8.66𝐸−01
Eph receptor A5	EphA5	−1.01	9.06𝐸−01
Integrin beta 1 (fibronectin receptor beta)	Itgb1	1.01	9.52𝐸−01
Transformation related protein 53; p53	Trp53	1.02	7.07𝐸−01
Endothelial cell-specific molecule 1	Esm1	1.03	6.89𝐸−01
Mitogen-activated protein kinase kinase 4; MKK4	Map2k4	1.04	7.06𝐸−01
Tissue inhibitor of metalloproteinases 3	Timp3	1.04	6.82𝐸−01
Eph receptor B2; Erk	Ephb2	1.05	7.47𝐸−01
CD82 antigen; Kai1	Cd82	1.06	4.01𝐸−01
Mitogen-activated protein kinase kinase 6; MKK6	Map2k6	1.07	3.79𝐸−01
5′ Nucleotidase, ecto; CD73	Nt5e	1.08	4.20𝐸−01
Epidermal growth factor receptor	Egfr	1.11	3.53𝐸−01
Phosphatidylinositol 3-kinase, catalytic, beta polypeptide	PI3K (Pik3cb)	1.12	3.09𝐸−01
Twist basic helix-loop-helix transcription factor 2	Twist2	1.13	1.30𝐸−01
Mediator complex subunit 23; CRSP3	Med23	1.15	7.53𝐸−02
Lysophosphatidic acid receptor 1; Edg2	Lpar1	1.19	1.46𝐸−02
Breast cancer metastasis-suppressor 1-like	Brms1l	1.27	2.76𝐸−02
GATA binding protein 3	Gata3	1.34	1.45𝐸−02
Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide	P4ha1	1.75	4.28𝐸−05

Genes with >2 times decrease or increase in expression and a P value < 0.05 were considered as significantly regulated. For Igf1r, Kiss1, and Hist1h2bk no data were available on the microarray.