480713.fig.001a
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480713.fig.001b
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480713.fig.001c
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480713.fig.001d
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Figure 1: Phospho-4E-BP1 levels and rapamycin-sensitive gene expression in murine OS cells. (a) Immunofluorescence analysis of K7M2 and K12 cells depicting phospho-4E-BP1 (p-4E-BP1) counterstained with DAPI. (b) Quantitative analysis of p-4E-BP1 staining comparing the percentage of positive K7M2 and K12 cells. (c) RT-PCR was performed on cellular RNA extracted from K7M2 and K12 cells in order to quantitate the relative expression of BMP2, BMP4, VEGF, and ALDH-1A1. GAPDH serves as a loading control. (d) RT-PCR was performed on cellular RNA extracted from K7M2 cells treated with rapamycin or DMSO only (K7M2) in order to quantitate the relative expression of BMP2, VEGF, and ALDH-1A1 in the presence of mTORC1 kinase inhibition. Again, GAPDH serves as a loading control. Asterisks (*) indicate statistically significant differences ( ).