480713.fig.002a
(a)
480713.fig.002b
(b)
480713.fig.002c
(c)
480713.fig.002d
(d)
480713.fig.002e
(e)
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(f)
Figure 2: Rapamycin treatment reduces ALDH activity and sensitizes K7M2 cells to oxidative stress. (a) ALDH activity was detected in K7M2 and K12 cells using FACS analysis and the relative amount of cells positive for ALDH is shown for each cell population. (b) K7M2 cells were treated with rapamycin or DMSO only (control) and analyzed by FACS as in (a). (c) K7M2 and K12 cells were treated with or without H2O2 (250 μM) and apoptosis was detected using PI staining. (d) A quantitative analysis of (c) illustrating the percentage of apoptotic cells after H2O2 treatment compared to untreated controls. (e) K7M2 cells were treated with or without H2O2, in the presence or absence (DMSO only control) of rapamycin, and apoptotic cells were detected as in (c). (f) A quantitative analysis of (e) illustrating the percentage of apoptotic cells after H2O2 compared to untreated controls. Asterisks (*) indicate statistically significant differences ( ).