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Figure 3: (a) Western blotting of MDM2, Akt, phosphoAkt, and β-actin. Total cell lysates were isolated 48 h after transfection. (b) Soft-agar colony formation assay (means ± SD, ). Black line shows significant difference ( ). (c) Relative proliferation rates of 10T1/2 cells and 10T1/2-H1047R cells transfected with MDM2 vector or empty vector, as measured by MTS assay (means ± SD, ).