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Volume 2013 (2013), Article ID 608964, 11 pages
Sustained Low-Dose Treatment with the Histone Deacetylase Inhibitor LBH589 Induces Terminal Differentiation of Osteosarcoma Cells
1Centre for Cancer Research, Monash Institute of Medical Research, Monash University, 27-31 Wright St, Clayton, VIC 3168, Australia
2Peter MacCallum Cancer Centre, St Andrews Place, East Melbourne, VIC 3002, Australia
3Andrew Love Cancer Centre, Deakin University, Barwon Health, 70 Swanston St, Geelong, VIC 3220, Australia
Received 31 December 2012; Accepted 25 January 2013
Academic Editor: C. Fisher
Copyright © 2013 Jason E. Cain et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Supplementary Figure 1: Effect of LBH589 in human osteosarcoma cells. Cell viability, western blot analysis and cell morphology in human osteosarcoma cells following 21 days culture in 15nM LBH589. A, B143 cell line. B, MG-63 cell line. C, Saos-2 cell line. D, SJSA cell line. Bar = 500 μm.
Supplementary Figure 2: Effect of LBH589 in human osteosarcoma cells on cell cycle and apoptosis. A, Phase contrast microscopy. B, Analysis of cell cycle by flow cytometry. C, Analysis of apoptosis by flow cytometry. Early apoptotic cells are represented by PI negative, Annexin V positive expression (bottom right quadrant). Bar = 500 μm.
Supplementary Figure 3: LBH589-mediated growth inhibition is irreversible. Cell viability following withdrawal of 15nM LBH589.
Supplementary Table 1: Primers for quantitative real-time PCR.
Supplementary Table 2: U2OS differentially expressed genes following 21-days culture (DMSO vehicle vs 15nM LBH589).
Supplementary Table 3: SJSA differentially expressed genes following 21-days culture (DMSO vehicle vs 15nM LBH589).
Supplementary Table 4: B143 differentially expressed genes following 21-days culture (DMSO vehicle vs 15nM LBH589).
Supplementary Table 5: U20S gene ontology analysis.