Genomic Breakpoint Characterization and Transcriptome Analysis of Metastatic, Recurrent Desmoplastic Small Round Cell Tumor

Figure 1

DSRCT validation: (a) H&E staining of primary and recurrent DSRCTs from the same patient (scale bar = 100 µm). (b) PCR amplification of the EWSR-WT1 fusion gene from cDNA derived from JN-DSRCT-1 and BER-DSRCT positive controls, the primary tumor (2911) and recurrent tumor (2912), or the 4JX1 negative control. (c) Histograms of Sanger sequencing from primary and recurrent DSRCTs show seamless transition between EWSR1 exon 7 and WT1 exon 8.

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