Genomic Breakpoint Characterization and Transcriptome Analysis of Metastatic, Recurrent Desmoplastic Small Round Cell Tumor

Figure 2

DSRCT genomic breakpoint map: (a) PCR strategy for amplifying the DSRCT genomic breakpoint using two different sets of EWSR1 forward primers with the same common set of WT1 reverse primers. (b) Histogram of Sanger sequencing results from the 291 patient’s tumors showing a 3 bp overlap at the EWSR1 intron 7 to WT1 intron 7 genomic breakpoint junction. (c) DSRCT genomic breakpoint map showing the breakpoint location and base pair overlap for 5 DSRCT cell lines and 7 DSRCTs.

(a)

(b)

(c)