Clinical Application of Human Mesenchymal Stromal Cells for Bone Tissue Engineering
Figure 6
Representative section of scaffold seeded with human bone marrow compared to that seeded with rat bone marrow. Calcium phosphate ceramic scaffolds were seeded with equal number of cells derived from either human or rat bone marrow and implanted subcutaneously in nude mice for 6 weeks. Almost all the pores of the scaffold seeded with rat cells are filled with newly formed bone while the pores of the scaffold seeded with human cells have only one pore with a small amount of bone while the rest of the pores are filled with fibrous tissue. The sections are stained with basic fuschin and methylene blue. The newly formed bone is stained red with basic fuschin (black arrows) while the remaining fibrous tissue is stained pink (white arrows). The black areas represent the scaffold.
