Acceleration of Functional Maturation and Differentiation of Neonatal Porcine Islet Cell Monolayers Shortly In Vitro Cocultured with Microencapsulated Sertoli Cells
Figure 8
(a) Western blot analysis of the indicated proteins from control NPI cell monolayers (C) or NPI cell monolayers cocultured with ESC (+ESC) for the indicated time periods. (b) The relative intensities of PDX-1 (open bars), Glut-2 (hatched bars), and GK (filled bars) levels, as determined by band densitometric analysis. The ratio of the band intensities was expressed as a percentage with respect to untreated control NPI cell monolayers. Data are shown as means ± SD from 3 samples. *Significant difference between two groups at each time point (). (c) Ratio insulin content/mg total protein of control NPI cell monolayers alone (open bars), or NPI cell monolayers, cocultivated with microencapsulated SC (gray bars), for 7, 14, and 21 days of culture. Data are shown as means ± SD from 3 samples.
