Plasticity of Cells and Ex Vivo Production of Red Blood Cells

Figure 4

In vitro enucleation of a MEDEP cell line, MEDEP-BRC5. The in vitro differentiation of MEDEP-BRC5 was performed as described in Figure 3. (A) Flow cytometric analyses. Before and After, the cells before and after in vitro differentiation. Percentages of propidium iodide-negative viable cells are shown. TER119, see Figure 3. SYTO16, a cell membrane-permeable fluorochrome dye to stain nucleic acids. Following in vitro differentiation, 52% of the cells were TER119-positive and SYTO16-negative cells, that is, the cells lacking nuclei. (B) Morphology of cells collected from the a, b, and c fractions shown in (A). Scale bars indicate 20 μm.