Human Motor Neuron Progenitor Transplantation Leads to Endogenous Neuronal Sparing in 3 Models of Motor Neuron Loss
Figure 1
hMNP differentiation in vitro. At day 28, GFAP+ astrocytes ((a) red) were rare within TUJ1+ ((a) green) cultures. The majority of TUJ1+ cells ((b, d) red) double stained for the motor neuron lineage marker Islet-1 ((b) green) and HB9 ((c, d) green). After 3 weeks of subsequent growth, cells displayed a mature, branched morphology consistent with mature motor neurons, and expressed the mature motor neuron markers SMI-32 ((e) green) and ChAT ((f) green). Counterstain in blue (a, d, e) and red (f). Bar = 50 μm.
