Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem Cells

Figure 3

Transcriptomic expression analyses of potential markers for primitive hMSC selection. Relative gene expression of hESC-derived hMSC (hES-MSC) or hMSC populations compared to hESC calibrator group. Messenger RNA levels were determined using QRT-PCR, and relative quantification (RQ) values were calculated by the 2^−ΔΔCt method with GAPDH as internal control and an arbitrary threshold at 35 cycles. Asterisks (∗) indicate significant expression variation with 𝑃 value < 0.05. As compared to hESC calibrator group, arrows pointing downwards (↓) indicate gene extinction (>35 Ct) and arrows pointing upwards (↑) indicate gene appearance (<35 Ct).

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