Human Fetal Liver: An In Vitro Model of Erythropoiesis

Figure 5

Gene expression of factors involved in definitive erythropoiesis. Quantitative real-time PCR analysis of genes known to be implied in definitive erythropoiesis at different times of culture (days 8, 11, 15, and 18). Relative gene expression is given by the 2^−ΔΔCt method where ΔΔCt represents the difference of an unknown sample (days 8, 11, 15, and 18 of culture) versus a control (day 0 of culture) for a target gene normalized to a control gene (GAPDH).