Research Article

An Inducible Expression System of the Calcium-Activated Potassium Channel 4 to Study the Differential Impact on Embryonic Stem Cells

Figure 1

Effects of doxycycline treatment on A2lox.cre ES cells. (a, b) SSEA-1 (a) and Oct4 (b) staining upon 3 days of treatment with 1 μg/mL of doxycycline revealed no difference in expression levels. (c) EBs were plated, and the percentage of EBs containing cardiac cell beating clusters was determined in dox-treated versus untreated cultures on day 10 after EB plating on day 4.
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