Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood

Table 2

Cloning efficiency of AB MNC and AB cell populations prospectively isolated on the basis of CD34/CD36 expression. Results are presented as mean (±SD) of those observed in three independent experiments. The inserts present the morphology of a representative BFU-E-derived colony obtained in the corresponding semisolid culture (original magnification 10x).

		CFC/plate				Total CFC per fraction*	Recovery
Cell population		BFU-E		CFU-GM	CFU-GEMM
	MNC; (10^5 cells/plate)		71±24	41±9	1±1	3.1×10^5	100%
	CD34^posCD36^neg; (500 cells/plate)		92±5	75±5	0.5±0.5	2.5×10^4	8.1%
	CD34^negCD36^neg; (10^5 cells/plate)		34±12	8±3	2±1	2.4×10^3	0.8%

* The total number of CFC per fraction was calculated by multiplying the frequency of CFC (BFU-E + CFU-GM + CFU-GEMM) per the total number of cells in the fraction presented in Table 1.