Research Article

Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells

Table 2

𝜙 C31 and SB chromosome junction sequences recovered from rat MAPC. Around 10 each of drug-resistant clones obtained with PhiC31 integrase and SB transposase were chosen at random to determine the site of genomic integration. Most clones showed more than one integration event, consistent with southern blot results. Exact genomic locations were determined by comparison of the chromosomal junction sequence with that of the rat genome.
(a)

𝜙 C31 Integrase
CloneChromosome bandLocation

14q42158791838
9q2251217205

22q44236122145
9q2251217122
15q1158645720
17q12.157593447

32q26141365031
3q2151055488
7q1332562140
15q1266145915
20q124538590

42q34s199022700
7q1332562264
9q2251217198
20q125244926

54q41139277121
6q1211098254
9q2251217202
Xq1218543448

617q12.157594096
Xq35134407720

71q41193493175
17q12.157593343
Xq35134407720

81q41193493175
9q2251217207

917q12.157593353

(b)

SB Transposase
CloneChromosome bandLocation

12q33171779390
13q1132001139
13q1139927168
20q1244445336

216p1413906741

31p1134991484
7q3294455924
9q3697344920

46q32128228570
8q2459559434
15q24104110283
16q12.157613884

55q35128927150
11q2377290939

63q35102093005
4q2259940513
4q2371432455
5q35134761715
16q12.260379119

76q1323449543
17q1149437069

87q1216815756
8q2477171774
9q117186249

99q3690439317
12p122500679

102q116206693
5q2148519577
7q1328888887
10q2675143457