Research Article

Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells

Table 3

Correlation of GFP expression and integration site for 𝜙 C31 Integrase. Three clones were selected based on the expression level of GFP (high, medium, or low). Plasmid rescue was carried out to determine if the expression level correlated with integrant number or genomic location.

Clone%GFPChromosome bandPosition

Lo<5%
<5%
<5%
5q12
17q12.1
Xq35
18035560
57593963
134407720

Med10–20%
10–20%
1q41
17q12.1
193493175
57594096

Hi>20%
>20%
>20%
>20%
8q31
10q31
17q12.1
Xq35
93775192
87259340
57594096
134407720