Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos

Figure 2

Characterization of CU1 hESC line. Immunohistochemical analysis with DAPI counterstain revealed that hESC colonies expressed pluripotency markers POU5F1/OCT4 (a), SSEA-4 (b), TRA1-60 (d), TRA-1-81 (e) (green fluorescence) and were negative for differentiation marker SSEA-1 (c). hESC cultured as embryoid bodies differentiated into all three germ layers. Hematoxylin and eosin (f), Masson’s trichrome (g), immunohistochemistry for neuron-specific beta-III tubulin (TUJ-1) (h), alpha fetoprotein (AFP) (i), and smooth muscle actin (SMA) (j) Scale bar = 100 μm.

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