Feline Neural Progenitor Cells I: Long-Term Expansion under Defined Culture Conditions

Figure 1

Morphology of cNPCs in different proliferation media at serial time points. Upper panel shows cNPCs cultured in SM and the lower panel cells of the same age in UM. Cultures are shown on day 0, after 1, 2, and 4 weeks as well as 4 months (UM only). In both media the cells exhibited morphologic features consistent with primitive neural cells throughout the period examined. In both cases, the adherent cells extended processes and expanded greatly during the initial week. The cells in SM ceased expanding during the first month and were not evaluated beyond the 3-month time point, whereas those in UM continued to expand throughout the course of the study. Scale bar: 100 μm.