Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media

Figure 3

Idealized growth response curve versus nutrient concentration illustrating the procedure for determining the “optimum” concentration of a nutrient. The range of concentrations that support optimum growth (referred to as a “plateau”) is determined, and its midpoint on the plot is selected as the concentration to be used in future media (Adapted from [92]).