Adult-Brain-Derived Neural Stem Cells Grafting into a Vein Bridge Increases Postlesional Recovery and Regeneration in a Peripheral Nerve of Adult Pig

Figure 7

GFP detection and phenotypic marker immunohistofluorescence (NeuN on left column, Schwann cells’ CNPase on right column) in sagittal sections of venous bridges which had been transplanted with LV-GFP-labeled neurospheres and were sampled at 240 days after lesion. (a, d) green endogenous fluorescence for detection of lineage-infected neurosphere progeny; (b, e) Alexa-594-revealed phenotypic marker immunoreactivities; (c, f) merged fluorescences. Note yellow-labeled colocalization of GFP-expressing neurosphere progeny with NeuN immunoreactivity (c) but not with CNPase (f). Scale bars: 0.5 mm.