Long-Term Cultured Human Term Placenta-Derived Mesenchymal Stem Cells of Maternal Origin Displays Plasticity

Figure 2

Pluripotency property of placental MSCs. (a) Oil red O staining (PhMSCsP5); (b) Von Kossa staining (PhMSCsP10); (c) “Falcon 25” static micromass cell culture system for chondrocyte differentiation; (d) saffranin O staining (PhMSCsP5); (e) hoechst 33342 staining of myotubules; (f) tubular assay; (g) neural differentiation of placental MSCs (PhMSCsP20); (h) map2 staining (PhMSCs021P15); (i) NeuN staining (PhMSCsP15); (j) GFAP staining (PhMSCs021P15); (k) Neural filament staining (PhMSCsP15); (l) Retinal cell differentiation of placental MSCs (PhMSCsP9); (m) Pancreatic progenitor cell differentiation of placental MSCs (PhMSCsP9) (n) dithizone (DTZ) positive pancreatic progenitor cells; (o) PCR analysis of ectodermal lineage (photoreceptor genes calbindin2 and recoverin) and endodermal lineage (pancreatic amylase gene).