Derivation of Neural Progenitors and Retinal Pigment Epithelium from Common Marmoset and Human Pluripotent Stem Cells

Figure 2

Transfection of cjes001 common marmoset CESCs and Riv9 HIPSCs. Micrographs (a) and FACS histograms (b) enumerating the percentage of eGFP-positive (eGFP +ve) cjes001 CESCs 24 hours after-transfection. (c) Numbers of drug-resistant and eGFP-expressing colonies formed after two weeks were scored for the stable transfection assay. (d) eGFP expression was lost in all pCMV-transfected clones. In contrast, all puromycin-resistant colonies were also eGFP +ve. Cjes001 (e′) and Riv9 clone (e′′) retained ubiquitous and constitutive eGFP expression while continuously express undifferentiated stem cell marker SSEA3 (red). Scale bars, 50 μm.