Derivation of Neural Progenitors and Retinal Pigment Epithelium from Common Marmoset and Human Pluripotent Stem Cells
Figure 3
Differentiation of cell progenitors associated with the central nervous system (CNS) and the neural retina. (a) Experimental overview for in vitro differentiation of CESCs. (b) Constitutive eGFP expression in differentiated aggregates of cjes001 EBs. (c) Q-PCR analysis of OCT4 and SOX2 pluripotency markers in undifferentiated cjes001 (0-day ESCs) and 7-day EBs. (d) Changes in morphology during in vitro differentiation. Arrowheads indicate EB outgrowth observed 1 week after replating. Neurites resembling neural progenitors (NPs) were formed 10–14 days after replating. Scale bars, 50 μm.
