Derivation of Neural Progenitors and Retinal Pigment Epithelium from Common Marmoset and Human Pluripotent Stem Cells
Figure 5
Differentiation of retinal pigmented epithelium (RPE) from Callithrix ESCs. (a) Stereoscopic image of cell outgrowth following EB replating. The white arrows indicate the visible pigmented area derived from an area of EB outgrowth. Black arrows indicate the colonies that did not develop to RPE structures. (b) Phase contrast and green fluorescence of the pigmented epithelium in RPE patch-like structures. The white arrowheads indicate the presence of putative RPE cells with typical pigmented cobblestone-like morphology. Scale bars, 200 μm. (c) Semiquantitative PCR analysis of manually picked clusters of pigmented epithelium (PE1 and PE2), nonpigmented cells (nPE), and undifferentiated ESCs. Water (H2O) only was included as negative control. (d) Relative expression levels of OCT4, PAX6, OTX2s and RPE65 mRNA in PE, nPE, embryoid bodies (EB), and undifferentiated ES cells (ES). Mean normalized expression of each target gene is relative to ACTB and GAPDH housekeeping genes. Error bars represent standard deviation. Asterisk shows significant difference of PAX6, OTX2, and RPE65 expression in PE clusters, .
