Nestin Protein Is Phosphorylated in Adult Neural Stem/Progenitor Cells and Not Endothelial Progenitor Cells

Table 1

Protein phosphorylation of adult CNS stem/progenitor cells, EPCs, and neoplastic ECs.

	pPeptides; (pPeptides/total peptides)	pSites			Multi-pPeptides
		(pSite/total pSites)			(single or multi-pSite peptides/total pPeptides)
		Serine	Threonine	Tyrosine	1p	2p	>2p
Neurospheres	495 (91.2%)	443 (84.2%)	71 (13.5%)	12 (2.3%)	401 (81.0%)	74 (14.9%)	20 (4.0%)
EPCs	250 ± 5 (59.7%)	194 ± 8 (87.4%)	20 ± 3 (9.0%)	8 ± 0 (3.6%)	228 ± 6 (91.0%)	19 ± 0 (7.4%)	4 ± 0 (1.6%)
Neoplastic ECs	980 (97.8%)	896 (88.1%)	103 (10.1%)	18 (1.8%)	675 (68.9%)	256 (26.1%)	49 (5.0%)

pPeptides, phosphopeptides; pSites, phosphorylation sites; multi-pPeptides, multi-phosphorylated peptides; 1p, single-phosphorylated site; 2p, two phosphorylated sites. Data are mean ± standard deviation.