Clonal Populations of Amniotic Cells by Dilution and Direct Plating: Evidence for Hidden Diversity
Figure 2
Flow cytometry of cell surface markers. Cell populations BMMSCp5, PB4A2p20, PB3B5p14, and PB1C4p15 were stained with antibodies against (a) CD73, CD29, CD44, SSEA4 and (b) against CD90 and CD105. Cell populations are indicated in vertical text on the left of the corresponding row of histograms for each marker. The markers and conjugated fluarochromes are indicated at the bottom of the corresponding column of each histogram. The -axis of all histograms corresponds to the mean florescence intensity (MFI) in log scale. The -axis is the percentage (%) of events in linear scale that were detected at each position of MFI along the -axis. Cell populations were gated to exclude presumptive dead cells and debris. The histogram of isotype controls is depicted by black line and the marker that is assayed is depicted in the filled histogram. 10,000 events were scored for all populations. The percentage of immunostained cells, excluding those stained by isotype controls, is indicated within each histogram.
