Temporal Requirements of cMyc Protein for Reprogramming Mouse Fibroblasts
Figure 1
Contribution of individual reprogramming factors to repression of Thy1. MEFs were infected with either all four reprogramming factors (OSMK), individual reprogramming factors (mOct4 or mSox2 or mcMyc, or mKlf4), or combinations of reprogramming factors (mcMyc & mKlf4, OS, OMK, SMK). On day 12 after infection, the percentage of cells expressing the fibroblast marker Thy1 was assessed by flow cytometry in all treatment groups. Mean percentage Thy1+ cells, normalized to untreated MEF controls (black bar) of experiments ± SEM are shown. Statistical significance established by one-way ANOVA, with “a” denoting groups insignificantly different from each other.
