Temporal Requirements of cMyc Protein for Reprogramming Mouse Fibroblasts
Figure 4
Thy1 downregulation by OSK, pTAT-mcMyc alone or OSK + pTAT-mcMyc at 5, 7, 9, and 12 days after infection. MEFs were infected with retrovirus harboring transgenes for mOct4, mSox2, and mKlf4 (OSK; orange line). 100 nM pTAT-mcMyc recombinant protein was applied to OSK expressing cells (red line), or to non-transduced MEFs (brown line). On days 5, 7, 9, and 12 after infection, percentage of cells expressing the fibroblast marker Thy1 was assessed in all treatment groups by flow cytometry with results normalized to untreated MEF controls (black line). Mean ± SEM of experiments shown.
