Temporal Requirements of cMyc Protein for Reprogramming Mouse Fibroblasts
Figure 5
A staggered approach to application of reprogramming factors. (a) MEF were pre-treated with mKlf4 (provirus) ± pTAT-mcMyc protein (or untreated) for 5 days and infected with mOct4 and mSox2 at day 0. Alternatively, MEF were not pre-treated and infected with mOct4+mSox2, mOct4+mSox2+mKlf4 or all four reprogramming factors (OSKM). The percentage of Thy1+ MEF was ascertained relative to untreated MEF at days 0, day 7 and day 14 post-infection. Statistical significance in percentage of Thy1+ MEF was assessed by one-way ANOVA. Mean ± SEM of experiments shown. (b) Oct4-GFP+ colony counts for experiments outlined in 6A were counted at days 0, 5, 7, 12, and 14 post-infection. Mean ± SEM of experiments shown.
