Stem Cells International

Research Article

Feline Neural Progenitor Cells II: Use of Novel Plasmid Vector and Hybrid Promoter to Drive Expression of Glial Cell Line-Derived Neurotrophic Factor Transgene

Figure 1

GFP expression in cNPCs transfected with pCAG-PyF101-eGFP plasmid. Transfected cNPC (passage 21) were maintained under proliferation conditions (UM) or switched to growth factor-free differentiation conditions (UM + 10% FBS) to evaluate potential loss of transgene expression. Cultures were photographed at 6, 7, 10, and 15 days. Sustained expression of green fluorescence protein (GFP) was observed for both conditions at all time points. Paired images are shown for each time point and include phase contrast (a, c, e, g, i, k, m, o) and fluorescence (b, d, f, h, j, l, n, p) micrographs of the same areas in culture.
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