Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells

<table>AF cells form gap junctions with cortical astrocytes <i>in vitro. </i>Immunocytochemistry showed that AF cells established gap junctions with cortical astrocytes. (a)–(c) CX43 (green) was expressed at the boundary between AF cells and GFAP- (red) positive cortical astrocytes. (d)–(f). In parallel experiments, AF cells labeled with an antibody against human-specific nuclear antigen (hNuc, green) showed discrete, punctuate CX43 (red) staining at the cellular boundary with cortical cells (arrows). (g)–(i). To determine the identity of cortical cells, similar cultures were stained with GFAP (yellow), hNuc (red), and Cx43 (green). Hoechst (blue) was used as a counter-stain. Scale bar: 8 <i>μ</i>m (a)–(c), 15 <i>μ</i>m (d)–(f), 20 <i>μ</i>m (g)–(i).</table>

Stem Cells International

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Figure 4: Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells 