The Potential of iPS Cells in Synucleinopathy Research
Figure 2
iPS cell-derived neurons express α-synuclein. (a) Immunofluorescence stainings of α-synuclein in TH (tyrosine hydroxylase) and TUBB3 (Tubulin beta-III) positive dopaminergic neurons after 5 months of differentiation show nuclear and vesicular localisation of α-synuclein, (b) and (c) RNA expression of α-synuclein (SNCA) and leucine-rich repeat kinase 2 (LRRK2) is upregulated in neurons compared to iPS cells and neural stem cells (NSCs) (normalized to the house-keeping gene HMBS), and (d) Subcellular fractionation of iPS cell-derived neurons shows nuclear (P1 fraction) and membrane associated, likely synaptic localization (P2 fraction) of α-synuclein.
