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Stem Cells International
Volume 2012 (2012), Article ID 646725, 9 pages
Skin-Derived Precursor Cells as an In Vitro Modelling Tool for the Study of Type 1 Neurofibromatosis
1Tissue Engineering Lab, Bioengineering Area, Instituto Biodonostia, Hospital Universitario Donostia, 20014 San Sebastián, Spain
2Department of Dermatology, Hospital Universitario Donostia, 20014 San Sebastián, Spain
Received 14 November 2011; Accepted 18 January 2012
Academic Editor: Mary Familari
Copyright © 2012 Araika Gutiérrez-Rivera et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The most characteristic feature of neurofibromatosis type 1 (NF1) is the development of neurofibromas. It has been suggested that these tumors are caused by somatic inactivation of the wild-type NF1 allele, but the cell that originally suffers this mutation remains controversial. Several lines of evidence support the clonal origin of these tumors, and it has been recently suggested that skin-derived precursor cells (SKPs) could be the cell of origin of dermal neurofibromas. Nullizygous (NF1−/−) SKPs do give rise to neurofibromas when transplanted to heterozygous mice. Moreover, a nullizygous population of cells that is S100β negative is present in human neurofibromas, and NF1+/− multipotent progenitor cells are seemingly recruited to the tumor. This evidence supports the neurofibroma stem cell hypothesis and a putative involvement of SKPs in the aetiopathogenesis of the disease, suggesting that SKPs could become a valuable tool for the in vitro study of NF1.
The tumor predisposition disorder von Recklinghausen’s neurofibromatosis type I (NF1) is one of the most common genetic disorders of the nervous system, affecting 1 in 3500 individuals worldwide [1–4]. The disease is caused by mutation in the NF1 gene (located on chromosome 17q11.2) that encodes the tumor suppressor protein neurofibromin, a GTPase-activating protein (GAP) [5, 6].
Neurofibromas are complex tumors that contain proliferating Schwann-like cells and other local supporting elements of the nerve fibers, as perineurial-like cells, fibroblasts, endothelial cells, pericytes, and vascular smooth muscle cells, as well as infiltration of mast cells . Although several reports have studied which cell originates this tumor, the present data are somewhat contradictory. In this paper we will address the issue of the cell of origin for dermal neurofibromas to explore if the available data support the cancer stem cell hypothesis. We will discuss recent findings in the light of possible involvement of the so-called skin-derived precursor cells in the aetiopathogenesis of this complex disease.
2. Skin-Derived Precursors (SKPs)
Skin-derived precursors (SKPs) are a population of neural crest-derived multipotent precursor cells present in both human and mouse dermis. They can be identified in vitro as nonadherent cells isolated from the dermis that proliferate and self-renew in response to growth factors FGF-2 and EGF. Under specific differentiation conditions, they give rise to progeny of the neuronal, glial, and mesodermal lineages [8–14].
SKPs thus derive from the dermis and apparently are distinct from mesenchymal stem cells and from central nervous system neural stem cells [8, 13], although they express genes characteristic of embryonic neural crest cells, such as Slug, Snail, Twist, Pax3, and Sox9 .
In vitro, SKPs can be differentiated into mesodermal lineages such as SMA+ smooth muscle cells and adipocytes, as well as into neural crest-derived tissues such as neurons and Schwann cells [8, 13]. In particular, SKPs give rise to cells with neuronal morphology that express the pan-neuronal markers βIII tubulin and neurofilament-M and proteins characteristic of peripheral neurons such as p75NTR, peripherin, NCAM, tyrosine hydroxylase, and dopamine β-hydroxylase. SKPs can also be differentiated into bipolar cells coexpressing glial fibrillary acidic protein GFAP, CNPase, S100β, and p75NTR, typical markers of cells with a differentiated Schwann phenotype, as well as MBP and P0 peripheral myelin protein [8, 13].
When transplanted in ovo into the chick neural crest migratory stream, SKPs mostly migrated into peripheral neural crest targets such as spinal nerve, dorsal root ganglia, and skin and expressed S100β . In vivo, it has recently been reported that SKPs derive from Sox2+ follicle-associated dermal precursors and show characteristics of dermal stem cells. In this respect, they contribute to dermal maintenance, wound healing, and hair follicle morphogenesis .
3. Type 1 Neurofibromatosis (NF1)
The primary clinical feature of NF1 is the development of benign peripheral nerve sheath tumors, termed neurofibromas . In a small percentage of NF1 patients, a particular type of neurofibromas (plexiform, see below) progress to malignant peripheral nerve sheath tumors (MPNSTs). NF1 patients are also predisposed to astrocytic brain tumors, pheochromocytoma, and juvenile myelomonocytic leukaemia [2, 17]. Noncancerous symptoms of the disease may include intellectual deficits, bone deformations, benign lesions of the iris (Lisch nodules), axillary freckling, and hyperpigmentation defects of the skin known as café-au-lait macules. Because many of the cardinal features of the disease affect neural crest-derived tissues, NF1 is considered as a neurocristopathy [16, 18–20].
NF1 is a dominantly inherited genetic disease. Half of the NF1 patients have inherited their NF1 mutation and the other half are caused by a de novo NF1 mutation, suggesting that the NF1 locus may represent a mutational hotspot in the human genome [3, 4, 16, 21]. Neurofibromin, the NF1 gene product, has a Ras GTPase activating (RasGAP) activity and negatively regulates Ras signaling [22, 23]. Neurofibromin functions as a tumor suppressor protein expressed in many cells although it is more abundant in cells from the nervous system such as neurons, Schwann cells, astrocytes, and oligodendrocytes as well as in leukocytes [6, 24, 25]. Loss or reduced neurofibromin expression leads to an increased Ras activity and it has been associated with increased mammalian target of rapamycin (mTOR) activity in astrocytes and Schwann cells [26, 27]. Moreover, neurofibromin plays a key role in the generation of cyclic AMP (cAMP) in both neurons and astrocytes [28, 29]. Loss of heterozygosity (LOH) in the inherited wild-type allele has been detected in some tumor types in NF1 patients, although it has been demonstrated that heterozygosity for NF1 is a key element for the development of many NF1 symptoms, including neurofibroma formation . An additional complexity of the disease is its variable phenotypic expression, suggesting that modifier genes and epigenetic phenomena may play an important role in disease manifestations .
4. Neurofibroma Subtypes and CellularComponents
The most common and complex feature of NF1 is the development of benign peripheral nerve sheath tumors or neurofibromas. Neurofibromas were classified by WHO into five subtypes : localized cutaneous, localized intraneural, plexiform, diffuse cutaneous, and soft tissue diffuse neurofibromas (elefantiasis neuromatosa).
Cutaneous neurofibromas reside exclusively in the skin and occur in virtually all individuals with NF1. They initially appear at puberty and increase in number with age and during pregnancy, suggesting a hormonal component in disease development [32–35]. These benign tumors, ranging from 0.1 to several cm in diameter, grow as discrete lesions in the dermis. Patients sometimes develop thousands of these tumors. Depending on their location, they can be painful and disfiguring for the patient and thus affect their quality of life. In contrast, plexiform neurofibromas develop internally along the plexus of major peripheral nerves and become quite large, sometimes involving an entire limb or body region . They occur in about 30% of the individuals and are thought to be congenital. While these tumors are also benign, they are debilitating and may progress to malignancy [37, 38]. The cellular make-up of these lesions is generally similar to that of dermal lesions.
In a physiological situation, a single peripheral nerve shaft is associated with myelinating or nonmyelinating Schwann cells. Several nerve fibers and associated Schwann cells are clustered into a nerve fascicle, each fascicle being surrounded by concentric layers of perineurial cells. Fibroblasts, endothelial cells, and occasional mast cells are also present in a normal nerve fascicle (Figure 1) [7, 16, 37, 38]. Neurofibromas contain all of the cell types found in normal peripheral nerve but in inappropriate numbers. Moreover, Schwann cells are found dissociated from nerves and the perineurium is often disrupted. Large amounts of intercellular collagen and ground substance are also typically present in neurofibromas .
5. How Many Mutagenic Events Are Needed for Neurofibromas to Arise?
Two types of congenital NF1 mutations have been found to influence neurofibroma number [40–42]. However, these two types of mutations affect only a small percentage of NF1 patients, and, moreover, patients bearing the same germline mutation can exhibit a very different number of dermal neurofibromas [43, 44], indicating that other mechanisms are implicated in neurofibroma formation.
Somatic mutations in the NF1 gene have been found in tumors associated with NF1, leading to functional loss of both alleles of the gene [45–47]. For example, loss of heterozygosity (LOH) in chromaffin cells initiates pheochromocytomas, and LOH in melanocytes produces pigmented lesions such as café-au-lait macules and Lisch nodules. LOH in myeloid cells induces myelomonocytic leukaemia, and LOH in glial cells permits astrocytoma formation [48–53].
It has also been suggested that neurofibromas are caused by somatic inactivation of the wild-type NF1 allele, leading to complete functional abrogation of the gene [45, 54, 55]. LOH in Schwann progenitor cells permits plexiform neurofibroma formation [30, 56], and it has been suggested that LOH in skin-derived precursors leads to cutaneous neurofibroma formation . Using both NF1 intragenic polymorphisms and markers from flanking and more distal regions of chromosome 17, Colman et al. demonstrated loss of heterozygosity (LOH) of the NF1 gene in eight neurofibromas from 22 patients and Serra et al. found LOH in 15 out of 60 dermal neurofibromas [55, 57]. Moreover, Sawada et al. identified a somatic deletion of the NF1 gene in a dermal neurofibroma with a defined germline mutation . LOH has also been detected in plexiform neurofibromas [58–60].
One possible explanation for the lack of allele loss detection in some tumors is that a more subtle somatic NF1 mutation occurred (point mutation, small deletion, insertion, or modification through epigenetic mechanisms). These changes do not produce loss of closely linked polymorphic marker loci . Alternatively, LOH may stay undetected because the presence of normal stromal or inflammatory tissue within the tumors increases sample background. Nevertheless, mechanisms that do not involve inactivation of the normal allele cannot be excluded. In dermal neurofibromas, local trauma can be a factor in the development of the tumors  and it has been suggested that dermal neurofibromas could be hyperplastic instead of neoplastic lesions, due to a poorly regulated wound healing in NF1 haploinsufficient tissues [63–65]. However most experts agree that these lesions are true neoplasms and are not hyperplastic.
6. Which Neurofibroma Cells Harbor Somatic NF1 Mutations?
Being a complex genetic disease with tumors of multicellular composition, the question arises which cell type within the tumor presents the secondary somatic mutations that characterize the pathological presentation of the dermal neurofibromas. Although NF1−/− fibroblasts exhibit greater proliferation capacity than their normal and heterozygous counterparts [63, 66], they are not normally found in tumors since only Schwann cells carry a double inactivation of the NF1 gene [59, 61, 67–69].
Two different populations of S100β+ cells (presumably terminally differentiated Schwann cells) have been demonstrated within in vitro cultures obtained from dermal neurofibromas, indicating that both NF1 Schwann cell subtypes (+/−) and (−/−) coexist in these tumors [61, 68]. This fact may be explained through two alternative possibilities: (i) the second hit mutation occurred as a secondary event within a neurofibroma that had already developed polyclonally, and thus only a subpopulation of S100β+ cells is (−/−), or (ii) the tumors arose through a two-hit mechanism within a stem/progenitor cell that gave rise to most tumor cells, but the proliferating neoplastic clone stimulated the proliferation of infiltrating nonneoplastic cells such as heterozygous Schwann cells, mast cells, and fibroblasts.
The influence of a heterozygous environment in plexiform neurofibroma development supports the latter theory. In a conditional plexiform neurofibroma mice model (NF1flox/−; Krox20cre), haploinsufficient stromal and mast cells (NF1+/−) are necessary and limiting for neurofibroma development [30, 70]. Accordingly, NF1−/− Schwann cell-derived secreted stem cell factor (SCF) causes a hyperactive recruitment of NF1+/− mast cells . Furthermore, NF1 mast cells secreted 2.5-fold higher TGFβ than wt mast cells, leading to a heightened fibroblast proliferation, migration, and collagen production . In all, these data reinforce the idea that heterozygous fibroblast and mast cells may play a key role in the neurofibroma pathogenesis .
7. Does Neurofibroma Originate from Stem/Progenitor Cells?
Cancer is a heterogeneous disease and tumors present a significant morphological, phenotypic, genetic, kinetic, and functional diversity. Several lines of evidence suggest that this heterogeneity could be due to a hierarchical organization of tumors that resembles normal tissue development. However another possible explanation is that tumor cells are biologically equivalent and that heterogeneity derives from extrinsic or intrinsic influences that result in stochastic responses . Strong evidence points to the importance of stem cells in the initiation and long-term maintenance of several cancers, as malignant germ cell cancers [74, 75], leukemias [76, 77], nervous system , breast  and colon cancers [80–83]. In these cancer types, several markers have been identified to distinguish the so called “cancer stem cells” that may form tumors when serially transplanted into immunocompromised NOD/SCID mice as compared to nontumorigenic cancer cells that do not present self-renewal capacities. Nevertheless, it is worth noting that in some cancers, most tumor cells fulfill this tumorigenic potential [84–86] and that the NOD/scid mouse transplantation assay sometimes might underestimate the frequency of human cancer cells with tumorigenic potential [85, 86].
The two-hit tumor suppressor hypothesis for NF1 predicts that all cells carry a constitutional mutation and a particular cell acquires a second mutation to initiate tumor formation . Based on the two-hit model of tumorigenesis, tumor cells in neurofibromas should be of clonal origin. Nevertheless, while both alleles are inactivated in NF1-associated malignancies, the clonal nature of the neurofibromas is controversial (see below) [58, 67, 88–91].
Interestingly, there is strong evidence that an adult multipotent stem/progenitor cell could be the cell of origin for cutaneous neurofibromas. It has been demonstrated that plexiform neurofibromas originate from embryonic neural crest-derived progenitors [30, 56, 92–96] and mice that develop plexiform tumors with 100% frequency fail to develop dermal tumors. Moreover, plexiform neurofibromas are congenital while cutaneous neurofibromas arise in puberty. The facts that dermal neurofibromas arise in the adulthood and locate in the dermis suggest the idea that dermal adult progenitor cells could be the source of these tumors . Furthermore, the close relationship observed between the development of cutaneous neurofibromas and hair follicle proximity suggests that adult progenitor cells residing in the hair follicle may be the origin of these tumors. There is evidence that the neurofibromas arise in the hair follicle vicinity and even small neurofibromas can be detected histologically in close contact with the hair follicle, in otherwise apparently healthy skin areas [97, 98]. Mechanical trauma has also been suggested to play a role in the pathogenesis of neurofibromas, that is, some neurofibromas appear to arise as a dysplastic response to crush trauma .
Several populations of stem/progenitor cells have been described to reside in the hair follicle or surrounding areas [81, 100–114], some of them being potential candidates for an involvement in NF1 pathogenesis. Recently, it has been speculated that recruitment of Nestin+ multipotent NF1+/− precursor cells is associated with cutaneous neurofibroma development . Histologically, nestin-positive small blood vessels and spindle-shaped tumor cells can be detected in the neurofibromas. In accordance with this hypothesis, S100β−/NF1− cells are detected in high proportion (16–31%) in neurofibromas. This fact could indicate the presence of multipotent stem cells that have suffered a second-hit mutation, although a dedifferentiation from S100β+/NF1− Schwann cells, also present in the tumor, can not be excluded .
Finally, there is strong evidence that SKPs could be the cell of origin for dermal neurofibromas . Cre-mediated recombination of SKPs induced in vitro loss of the wt allele in these cells. When transplanted into the same mice that originated these cells, SKPs (but not control cells) then initiated dermal neurofibromas. However, tumor formation was only efficient in female recipients that were pregnant at the time of implantation, highlighting the hormone sensitivity observed in patients and the importance of the microenvironment during neurofibroma formation. Furthermore, deletion of in the skin of CMV-CreERt2 mice after topical application of tamoxifen led to local dermal neurofibroma formation, supporting the notion that the cell of origin for these tumors resides within the skin at close range of topical tamoxifen application [34, 115].
8. SKPs as a Tool for In Vitro Modelling of NF1 Features
Several lines of evidence now point to a stem cell origin of dermal neurofibromas. On the one hand, a number of studies have assessed the clonal origin of neurofibromas, based on X chromosome inactivation (XCI) clonality assay. In our view, the results are still controversial since (i) clonal cell origin may not formally be proven through XCI analyses and (ii) studies have generally been performed with low patient numbers. For instance, Skuse et al. studied eight dermal neurofibromas and concluded that all of them were of clonal origin . Tucker et al. also found evidence for clonality in some of the six neurofibromas studied , suggesting that although other mechanisms could be at stake, at least in some neurofibromas a unique stem cell may have suffered a second-hit mutation, giving rise to a nullizygous Schwann cell progeny. On the other hand, only one kind of somatic mutation has been found in every neurofibroma analyzed and different neurofibromas of the same patient present different somatic mutations [61, 68], reinforcing the neurofibroma stem cell hypothesis. Moreover, multipotent stem cells (termed neurofibroma-derived precursor cells or NFPs) have been isolated from dermal neurofibromas. These precursors express Nestin and show a multipotent differentiation potential, giving rise to Schwann cells, neurons, epithelial cells, and adipocites . However NFPs do not contain the somatic NF1 mutation and thus their relationship with NF1 pathogenesis is currently unclear. Similarly, characterization of cells present in neurofibromas by S100β, a marker for the Schwann lineage, has demonstrated that a nullizygous population (NF1−/−) that is negative for S100β expression is present in neurofibromas. Although it cannot be discarded that they could be dedifferentiated Schwann cells, it is also possible that they could be progenitor cells that have suffered the somatic mutation and that generate the Schwann cells present in the tumor. In any case, cell characterization by a single marker is less than optimal and too many interpretations of these results are possible as to extract any meaningful conclusion.
Recently, an elegant study showed that NF1+/− SKPs could form neurofibromas in a conditional mouse model, although a key role for tumor environment was also found . To date there is no data on involvement of SKPs in human neurofibroma development, although NF1+/− multipotent progenitor cells are supposedly recruited to form dermal neurofibromas . If SKPs were the cells of origin of dermal neurofibromas, NF1−/− SKPs should be present within NF1 patient neurofibromas, although NF1+/− SKPs should also be detected. If these putative NF1−/− SKPs would present a predisposition to differentiate preferentially into the Schwann cell lineage should also be explored (Figure 2).
In summary, current evidence supports the notion that, at least in murine models, skin-derived precursor cells (SKPs) might be a cell of origin for dermal neurofibromas. It is also conceivable that human SKPs might be the cell of origin of neurofibromas, although formal proof for this is lacking. Isolation of SKPs from human neurofibromas could demonstrate if these dermal multipotent stem cells bear the somatic mutation and whether or not this mutation confers a predisposition to these precursor cells to differentiate into the Schwann cell lineage. Furthermore, isolation of SKPs from healthy skin of NF1 patients could demonstrate if there are SKPs with the somatic mutation, even in areas where the neurofibroma is histologically undetectable. In conclusion, SKPs may become a useful tool for the in vitro study of the neurofibromatosis type 1 syndrome.
This work was financed by grants provided by MICINN-Instituto de Salud Carlos III (Proyecto Inv. Salud, PI10/02871) and Gobierno Vasco (Dpto. de Industria, SAIO10-PE10BF01). A. Izeta was supported by the “Programa I3SNS” (CES09/015) from Instituto de Salud Carlos III and by Osakidetza-Servicio Vasco de Salud (Spain).
- J. M. Friedman, “Epidemiology of neurofibromatosis type 1,” American Journal of Medical Genetics, vol. 89, no. 1, pp. 1–6, 1999.
- V. M. Riccardi, Neurofibromatosis: phenotype, Natural history, and Pathogenesis, The Johns Hopkins University Press, Baltimore, Md, USA, 2nd edition, 1992.
- J. Szudek, P. Birch, V. M. Riccardi, D. G. Evans, and J. M. Friedman, “Associations of clinical features in neurofibromatosis 1 (NF1),” Genetic Epidemiology, vol. 19, no. 4, pp. 429–439, 2000.
- A. B. Trovó-Marqui and E. H. Tajara, “Neurofibromin: a general outlook,” Clinical Genetics, vol. 70, no. 1, pp. 1–13, 2006.
- J. E. DeClue, B. D. Cohen, and D. R. Lowy, “Identification and characterization of the neurofibromatosis type 1 protein product,” Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 22, pp. 9914–9918, 1991.
- D. H. Gutmann, D. L. Wood, and F. S. Collins, “Identification of the neurofibromatosis type 1 gene product,” Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 21, pp. 9658–9662, 1991.
- M. Kimura, Y. Kamata, K. Matsumoto, and H. Takaya, “Electron microscopical study on the tumor of Von Recklinghausen's neurofibromatosis,” Acta Pathologica Japonica, vol. 24, no. 1, pp. 79–91, 1974.
- K. J. L. Fernandes, I. A. McKenzie, P. Mill et al., “A dermal niche for multipotent adult skin-derived precursor cells,” Nature Cell Biology, vol. 6, no. 11, pp. 1082–1093, 2004.
- K. J. L. Fernandes, J. G. Toma, and F. D. Miller, “Multipotent skin-derived precursors: adult neural crest-related precursors with therapeutic potential,” Philosophical Transactions of the Royal Society B, vol. 363, no. 1489, pp. 185–198, 2008.
- N. Gago, V. Pérez-López, J. P. Sanz-Jaka et al., “Age-dependent depletion of human skin-derived progenitor cells,” Stem Cells, vol. 27, no. 5, pp. 1164–1172, 2009.
- D. P. J. Hunt, C. Jahoda, and S. Chandran, “Multipotent skin-derived precursors: from biology to clinical translation,” Current Opinion in Biotechnology, vol. 20, no. 5, pp. 522–530, 2009.
- I. A. McKenzie, J. Biernaskie, J. G. Toma, R. Midha, and F. D. Miller, “Skin-derived precursors generate myelinating Schwann cells for the injured and dysmyelinated nervous system,” Journal of Neuroscience, vol. 26, no. 24, pp. 6651–6660, 2006.
- J. G. Toma, M. Akhavan, K. J. L. Fernandes et al., “Isolation of multipotent adult stem cells from the dermis of mammalian skin,” Nature Cell Biology, vol. 3, no. 9, pp. 778–784, 2001.
- J. G. Toma, I. A. McKenzie, D. Bagli, and F. D. Miller, “Isolation and characterization of multipotent skin-derived precursors from human skin,” Stem Cells, vol. 23, no. 6, pp. 727–737, 2005.
- J. Biernaskie, M. Paris, O. Morozova et al., “SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells,” Cell Stem Cell, vol. 5, no. 6, pp. 610–623, 2009.
- K. Cichowski and T. Jacks, “NF1 tumor suppressor gene function: narrowing the GAP,” Cell, vol. 104, no. 4, pp. 593–604, 2001.
- J. L. Bader, “Neurofibromatosis and cancer,” Annals of the New York Academy of Sciences, vol. 486, pp. 57–65, 1986.
- L. Q. Le and L. F. Parada, “Tumor microenvironment and neurofibromatosis type I: connecting the GAPs,” Oncogene, vol. 26, no. 32, pp. 4609–4616, 2007.
- B. A. Ward and D. H. Gutmann, “Neurofibromatosis 1: from lab bench to clinic,” Pediatric Neurology, vol. 32, no. 4, pp. 221–228, 2005.
- Y. Zhu and L. F. Parada, “Neurofibromin, a tumor suppressor in the nervous system,” Experimental Cell Research, vol. 264, no. 1, pp. 19–28, 2001.
- S. M. H. Hughes, The Neurofibromatoses: A Pathogenic and Clinical Overview, Chapman & Hall, London, UK, 1994.
- R. Ballester, D. Marchuk, M. Boguski et al., “The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins,” Cell, vol. 63, no. 4, pp. 851–859, 1990.
- G. Xu, P. O'Connell, D. Viskochil et al., “The neurofibromatosis type 1 gene encodes a protein related to GAP,” Cell, vol. 62, no. 3, pp. 599–608, 1990.
- M. M. Daston and N. Ratner, “Neurofibromin, a predominantly neuronal GTPase activating protein in the adult, is ubiquitously expressed during development,” Developmental Dynamics, vol. 195, no. 3, pp. 216–226, 1993.
- M. M. Daston, H. Scrable, M. Nordlund, A. K. Sturbaum, L. M. Nissen, and N. Ratner, “The protein product of the neurofibromatosis type 1 gene is expressed at highest abundance in neurons, Schwann cells, and oligodendrocytes,” Neuron, vol. 8, no. 3, pp. 415–428, 1992.
- B. Dasgupta, Y. Yi, D. Y. Chen, J. D. Weber, and D. H. Gutmann, “Proteomic analysis reveals hyperactivation of the mammalian target of rapamycin pathway in neurofibromatosis 1-associated human and mouse brain tumors,” Cancer Research, vol. 65, no. 7, pp. 2755–2760, 2005.
- C. M. Johannessen, E. E. Reczek, M. F. James, H. Brems, E. Legius, and K. Cichowski, “The NF1 tumor suppressor critically regulates TSC2 and mTOR,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 24, pp. 8573–8578, 2005.
- B. Dasgupta, L. L. Dugan, and D. H. Gutmann, “The neurofibromatosis 1 gene product neurofibromin regulates pituitary adenylate cyclase-activating polypeptide-mediated signaling in astrocytes,” Journal of Neuroscience, vol. 23, no. 26, pp. 8949–8954, 2003.
- J. Tong, F. Hannan, Y. Zhu, A. Bernards, and Y. Zhong, “Neurofibromin regulates G protein-stimulated adenylyl cyclase activity,” Nature Neuroscience, vol. 5, no. 2, pp. 95–96, 2002.
- Y. Zhu, P. Ghosh, P. Charnay, D. K. Burns, and L. F. Parada, “Neurofibromas in NF1: schwann cell origin and role of tumor environment,” Science, vol. 296, no. 5569, pp. 920–922, 2002.
- B. W. Scheithauer, J. M. Woodruff, and C. R. Antonescu, “Perineurioma,” in WHO Classification of Tumours of the Central Nervous System, D. N. Louis, et al., Ed., pp. 158–159, International Agency for Research on Cancer (IARC), Lyon, France, 2007.
- R. E. Ferner, “Neurofibromatosis 1,” European Journal of Human Genetics, vol. 15, no. 2, pp. 131–138, 2007.
- M. M. Lakkis and G. I. Tennekoon, “Neurofibromatosis type 1 I. General overview,” Journal of Neuroscience Research, vol. 62, no. 6, pp. 755–763, 2000.
- L. Q. Le, T. Shipman, D. K. Burns, and L. F. Parada, “Cell of origin and microenvironment contribution for NF1-associated dermal neurofibromas,” Cell Stem Cell, vol. 4, no. 5, pp. 453–463, 2009.
- T. M. Roth, E. M. Petty, and K. F. Barald, “The role of steroid hormones in the NF1 phenotype: focus on pregnancy,” American Journal of Medical Genetics, Part A, vol. 146, no. 12, pp. 1624–1633, 2008.
- B. R. Korf, “Plexiform neurofibromas,” American Journal of Medical Genetics, vol. 89, no. 1, pp. 31–37, 1999.
- P. Lévy, D. Vidaud, K. Leroy et al., “Molecular profiling of malignant peripheral nerve sheath tumors associated with neurofibromatosis type 1, based on large-scale real-time RT-PCR,” Molecular Cancer, vol. 3, article 20, 2004.
- J. M. Woodruff, “Pathology of tumors of the peripheral nerve sheath in type 1 neurofibromatosis,” American Journal of Medical Genetics, vol. 89, no. 1, pp. 23–30, 1999.
- V. M. Riccardi and R. A. Lewis, “Penetrance of von Recklinghausen neurofibromatosis: a distinction between predecessors and descendants,” American Journal of Human Genetics, vol. 42, no. 2, pp. 284–289, 1988.
- L. M. Kayes, W. Burke, V. M. Riccardi et al., “Deletions spanning the neurofibromatosis I gene: identification and phenotype of five patients,” American Journal of Human Genetics, vol. 54, no. 3, pp. 424–436, 1994.
- M. Upadhyaya, S. M. Huson, M. Davies et al., “An absence of cutaneous neurofibromas associated with a 3-bp inframe deletion in exon 17 of the NF1 gene (c.2970-2972 delAAT): evidence of a clinically significant NF1 genotype-phenotype correlation,” American Journal of Human Genetics, vol. 80, no. 1, pp. 140–151, 2007.
- B. L. Wu, M. A. Austin, G. H. Schneider, R. G. Boles, and B. R. Korf, “Deletion of the entire NF1 gene detected by fish: four deletion patients associated with severe manifestations,” American Journal of Medical Genetics, vol. 59, no. 4, pp. 528–535, 1995.
- E. Ars, E. Serra, J. García et al., “Mutations affecting mRNA splicing are the most common molecular defects in patients with neurofibromatosis type 1,” Human Molecular Genetics, vol. 9, no. 2, pp. 237–247, 2000.
- J. C. Carey, J. M. Laub, and B. D. Hall, “Penetrance and variability in neurofibromatosis: a genetic study of 60 families,” Birth Defects, vol. 15, no. 5, pp. 271–281, 1979.
- T. De Raedt, O. Maertens, M. Chmara et al., “Somatic loss of wild type NF1 allele in neurofibromas: comparison of NF1 microdeletion and non-microdeletion patients,” Genes Chromosomes and Cancer, vol. 45, no. 10, pp. 893–904, 2006.
- E. Legius, D. A. Marchuk, F. S. Collins, and T. W. Glover, “Somatic deletion of the neurofibromatosis type 1 gene in a neurofibrosarcoma supports a tumour suppressor gene hypothesis,” Nature Genetics, vol. 3, no. 2, pp. 122–126, 1993.
- Y. Li, G. Bollag, R. Clark et al., “Somatic mutations in the neurofibromatosis 1 gene in human tumors,” Cell, vol. 69, no. 2, pp. 275–281, 1992.
- M. L. Bajenaru, M. R. Hernandez, A. Perry et al., “Optic nerve glioma in mice requires astrocyte NF1 gene inactivation and NF1 brain heterozygosity,” Cancer Research, vol. 63, no. 24, pp. 8573–8577, 2003.
- S. De Schepper, O. Maertens, T. Callens, J. M. Naeyaert, J. Lambert, and L. Messiaen, “Somatic mutation analysis in NF1 café au lait spots reveals two NF1 hits in the melanocytes,” Journal of Investigative Dermatology, vol. 128, no. 4, pp. 1050–1053, 2008.
- D. A. Largaespada, C. I. Brannan, N. A. Jenkins, and N. G. Copeland, “NF1 deficiency causes Ras-mediated granulocyte/macrophage colony stimulating factor hypersensitivity and chronic myeloid leukaemia,” Nature Genetics, vol. 12, no. 2, pp. 137–143, 1996.
- F. Li, A. M. Munchhof, H. A. White et al., “Neurofibromin is a novel regulator of RAS-induced signals in primary vascular smooth muscle cells,” Human Molecular Genetics, vol. 15, no. 11, pp. 1921–1930, 2006.
- A. M. Munchhof, F. Li, H. A. White et al., “Neurofibroma-associated growth factors activate a distinct signaling network to alter the function of neurofibromin-deficient endothelial cells,” Human Molecular Genetics, vol. 15, no. 11, pp. 1858–1869, 2006.
- Y. Zhu, T. Harada, L. Liu et al., “Inactivation of NF1 in CNS causes increased glial progenitor proliferation and optic glioma formation,” Development, vol. 132, no. 24, pp. 5577–5588, 2005.
- S. Sawada, S. Florell, S. M. Purandare, M. Ota, K. Stephens, and D. Viskochil, “Identification of NF1 mutations in both alleles of a dermal neurofibroma,” Nature Genetics, vol. 14, no. 1, pp. 110–112, 1996.
- E. Serra, S. Puig, D. Otero et al., “Confirmation of a double-hit model for the NF1 gene in benign neurofibromas,” American Journal of Human Genetics, vol. 61, no. 3, pp. 512–519, 1997.
- L. Q. Le, C. Liu, T. Shipman, Z. Chen, U. Suter, and L. F. Parada, “Susceptible stages in Schwann cells for NF1-associated plexiform neurofibroma development,” Cancer Research, vol. 71, no. 13, pp. 4686–4695, 2011.
- S. D. Colman, C. A. Williams, and M. R. Wallace, “Benign neurofibromas in type 1 neurofibromatosis (NF1) show somatic deletions of the NF1 gene,” Nature Genetics, vol. 11, no. 1, pp. 90–92, 1995.
- K. Däschner, G. Assum, I. Eisenbarth et al., “Clonal origin of tumor cells in a plexiform neurofibroma with LOH in NF1 intron 38 and in dermal neurofibromas without LOH of the NF1 gene,” Biochemical and Biophysical Research Communications, vol. 234, no. 2, pp. 346–350, 1997.
- L. Kluwe, R. E. Friedrich, and V. F. Mautner, “Allelic loss of the NF1 gene in NF1-associated plexiform neurofibromas,” Cancer Genetics and Cytogenetics, vol. 113, no. 1, pp. 65–69, 1999.
- S. A. Rasmussen, J. Overman, S. A. M. Thomson et al., “Chromosome 17 loss-of-heterozygosity studies in benign and malignant tumors in neurofibromatosis type 1,” Genes Chromosomes and Cancer, vol. 28, no. 4, pp. 425–431, 2000.
- O. Maertens, H. Brems, J. Vandesompele et al., “Comprehensive NF1 screening on cultured Schwann cells from neurofibromas,” Human Mutation, vol. 27, no. 10, pp. 1030–1040, 2006.
- V. M. Riccardi, “Of mass and men: neurofibromas and histogenesis,” Archives of Dermatology, vol. 136, no. 10, pp. 1257–1258, 2000.
- R. P. Atit, M. J. Crowe, D. G. Greenhalgh, R. J. Wenstrup, and N. Ratner, “The Nf1 tumor suppressor regulates mouse skin wound healing, fibroblast proliferation, and collagen deposited by fibroblasts,” Journal of Investigative Dermatology, vol. 112, no. 6, pp. 835–842, 1999.
- T. Tucker and J. M. Friedman, “Pathogenesis of hereditary tumors: beyond the “two-hit” hypothesis,” Clinical Genetics, vol. 62, no. 5, pp. 345–357, 2002.
- H. Ylä-Outinen, V. Aaltonen, A. S. Björkstrand et al., “Upregulation of tumor suppressor protein neurofibromin in normal human wound healing and in vitro evidence for platelet derived growth factor (PDGF) and transforming growth factor-β1 (TGF-β1) elicited increase in neurofibromin mRNA steady-state levels in dermal fibroblasts,” Journal of Investigative Dermatology, vol. 110, no. 3, pp. 232–237, 1998.
- T. Rosenbaum, Y. L. Boissy, K. Kombrinck et al., “Neurofibromin-deficient fibroblasts fail to form perineurium in vitro,” Development, vol. 121, no. 11, pp. 3583–3592, 1995.
- J. L. Rutkowski, K. Wu, D. H. Gutmann, P. J. Boyer, and E. Legius, “Genetic and cellular defects contributing to benign tumor formation in neurofibromatosis type 1,” Human Molecular Genetics, vol. 9, no. 7, pp. 1059–1066, 2000.
- E. Serra, T. Rosenbaum, U. Winner et al., “Schwann cells harbor the somatic NF1 mutation in neurofibromas: evidence of two different Schwann cell subpopulations,” Human Molecular Genetics, vol. 9, no. 20, pp. 3055–3064, 2000.
- L. S. Sherman, R. Atit, T. Rosenbaum, A. D. Cox, and N. Ratner, “Single cell Ras-GTP analysis reveals altered Ras activity in a subpopulation of neurofibroma Schwann cells but not fibroblasts,” The Journal of Biological Chemistry, vol. 275, no. 39, pp. 30740–30745, 2000.
- F. C. Yang, D. A. Ingram, S. Chen et al., “Nf1-dependent tumors require a microenvironment containing and c-kit-dependent bone marrow,” Cell, vol. 135, no. 3, pp. 437–448, 2008.
- F. C. Yang, D. A. Ingram, S. Chen et al., “Neurofibromin-deficient Schwann cells secrete a potent migratory stimulus for mast cells,” The Journal of Clinical Investigation, vol. 112, no. 12, pp. 1851–1861, 2003.
- F. C. Yang, S. Chen, T. Clegg et al., “ mast cells induce neurofibroma like phenotypes through secreted TGF-β signaling,” Human Molecular Genetics, vol. 15, no. 16, pp. 2421–2437, 2006.
- J. E. Dick, “Stem cell concepts renew cancer research,” Blood, vol. 112, no. 13, pp. 4793–4807, 2008.
- K. Illmensee and B. Mintz, “Totipotency and normal differentiation of single teratocarcinoma cells cloned by injection into blastocysts,” Proceedings of the National Academy of Sciences of the United States of America, vol. 73, no. 2, pp. 549–553, 1976.
- L. J. Kleinsmith and G. B. Pierce Jr., “Multipotentiality of single embryonal carcinoma cells,” Cancer Research, vol. 24, pp. 1544–1551, 1964.
- D. Bonnet and J. E. Dick, “Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell,” Nature Medicine, vol. 3, no. 7, pp. 730–737, 1997.
- T. Lapidot, C. Sirard, J. Vormoor et al., “A cell initiating human acute myeloid leukaemia after transplantation into SCID mice,” Nature, vol. 367, no. 6464, pp. 645–648, 1994.
- S. K. Singh, I. D. Clarke, T. Hide, and P. B. Dirks, “Cancer stem cells in nervous system tumors,” Oncogene, vol. 23, no. 43, pp. 7267–7273, 2004.
- M. Al-Hajj, M. S. Wicha, A. Benito-Hernandez, S. J. Morrison, and M. F. Clarke, “Prospective identification of tumorigenic breast cancer cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 7, pp. 3983–3988, 2003.
- P. Dalerba and M. F. Clarke, “Cancer stem cells and tumor metastasis: first steps into uncharted territory,” Cell Stem Cell, vol. 1, no. 3, pp. 241–242, 2007.
- V. Horsley, D. O'Carroll, R. Tooze et al., “A human colon cancer cell capable of initiating tumour growth in immunodeficient mice,” Cell, vol. 126, no. 3, pp. 597–609, 2006.
- C. A. O'Brien, A. Pollett, S. Gallinger, and J. E. Dick, “A human colon cancer cell capable of initiating tumour growth in immunodeficient mice,” Nature, vol. 445, no. 7123, pp. 106–110, 2007.
- L. Ricci-Vitiani, D. G. Lombardi, E. Pilozzi et al., “Identification and expansion of human colon-cancer-initiating cells,” Nature, vol. 445, no. 7123, pp. 111–115, 2007.
- P. N. Kelly, A. Dakic, J. M. Adams, S. L. Nutt, and A. Strasser, “Tumor growth need not be driven by rare cancer stem cells,” Science, vol. 317, no. 5836, p. 337, 2007.
- E. Quintana, M. Shackleton, M. S. Sabel, D. R. Fullen, T. M. Johnson, and S. J. Morrison, “Efficient tumour formation by single human melanoma cells,” Nature, vol. 456, no. 7222, pp. 593–598, 2008.
- M. Shackleton, E. Quintana, E. R. Fearon, and S. J. Morrison, “Heterogeneity in cancer: cancer stem cells versus clonal evolution,” Cell, vol. 138, no. 5, pp. 822–829, 2009.
- A. G. Knudson Jr., “Overview: genes that predispose to cancer,” Mutation Research, vol. 247, no. 2, pp. 185–190, 1991.
- I. Eisenbarth, K. Beyer, W. Krone, and G. Assum, “Toward a survey of somatic mutation of the NF1 gene in benign neurofibromas of patients with neurofibromatosis type 1,” American Journal of Human Genetics, vol. 66, no. 2, pp. 393–401, 2000.
- P. J. Fialkow, R. W. Sagebiel, S. M. Gartler, and D. L. Rimoin, “Multiple cell origin of hereditary neurofibromas,” The New England Journal of Medicine, vol. 284, no. 6, pp. 298–300, 1971.
- G. R. Skuse, B. A. Kosciolek, and P. T. Rowley, “The neurofibroma in von Recklinghausen neurofibromatosis has a unicellular origin,” American Journal of Human Genetics, vol. 49, no. 3, pp. 600–607, 1991.
- T. Tucker, V. M. Riccardi, C. Brown et al., “S100B and neurofibromin immunostaining and X-inactivation patterns of laser-microdissected cells indicate a multicellular origin of some NF1-associated neurofibromas,” Journal of Neuroscience Research, vol. 89, no. 9, pp. 1451–1460, 2011.
- K. Cichowski, T. S. Shih, E. Schmitt et al., “Mouse models of tumor development in neurofibromatosis type 1,” Science, vol. 286, no. 5447, pp. 2172–2176, 1999.
- N. M. Joseph, J. T. Mosher, J. Buchstaller et al., “Mouse tumor model for neurofibromatosis type 1,” Cancer Cell, vol. 13, no. 2, pp. 129–140, 2008.
- K. S. Vogel, L. J. Klesse, S. Velasco-Miguel, K. Meyers, E. J. Rushing, and L. F. Parada, “Mouse tumor model for neurofibromatosis type 1,” Science, vol. 286, no. 5447, pp. 2176–2179, 1999.
- J. Wu, J. P. Williams, T. A. Rizvi et al., “Plexiform and dermal neurofibromas and pigmentation are caused by Nf1 loss in desert hedgehog-expressing cells,” Cancer Cell, vol. 13, no. 2, pp. 105–116, 2008.
- H. Zheng, L. Chang, N. Patel et al., “Induction of abnormal proliferation by nonmyelinating schwann cells triggers neurofibroma formation,” Cancer Cell, vol. 13, no. 2, pp. 117–128, 2008.
- E.-M. Jouhilahti, S. Peltonen, T. Callens et al., “The development of cutaneous neurofibromas,” American Journal of Pathology, vol. 178, no. 2, pp. 500–505, 2011.
- S. L. Karvonen, M. Kallioinen, H. Ylä-Outinen, M. Pöyhönen, A. Oikarinen, and J. Peltonen, “Occult neurofibroma and increased S100 protein in the skin of patients with neurofibromatosis type 1: new insight to the etiopathomechanism of neurofibromas,” Archives of Dermatology, vol. 136, no. 10, pp. 1207–1209, 2000.
- V. M. Riccardi, “Neurofibromatosis type 1 is a disorder of dysplasia: the importance of distinguishing features, consequences, and complications,” Birth Defects Research Part A, vol. 88, no. 1, pp. 9–14, 2010.
- C. Blanpain, W. E. Lowry, A. Geoghegan, L. Polak, and E. Fuchs, “Self-renewal, multipotency, and the existence of two cell populations within an epithelial stem cell niche,” Cell, vol. 118, no. 5, pp. 635–648, 2004.
- S. Claudinot, M. Nicolas, H. Oshima, A. Rochat, and Y. Barrandon, “Long-term renewal of hair follicles from clonogenic multipotent stem cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 41, pp. 14677–14682, 2005.
- G. Cotsarelis, T. T. Sun, and R. M. Lavker, “Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis,” Cell, vol. 61, no. 7, pp. 1329–1337, 1990.
- A. Gutiérrez-Rivera, A. Pavón-Rodríguez, P. Cormenzana, J. P. Sanz-Jaka, and A. Izeta, “A protocol for enrichment of CD34+ stromal cell fraction through human skin disaggregation and magnetic separation,” Journal of Dermatological Science, vol. 59, no. 1, pp. 60–62, 2010.
- A. Gutiérrez-Rivera, A. Pavón-Rodríguez, F. Jiménez-Acosta et al., “Functional characterization of highly adherent CD34+ keratinocytes isolated from human skin,” Experimental Dermatology, vol. 19, no. 7, pp. 685–688, 2010.
- A. Haegebarth and H. Clevers, “Wnt signaling, Lgr5, and stem cells in the intestine and skin,” American Journal of Pathology, vol. 174, no. 3, pp. 715–721, 2009.
- V. Jaks, N. Barker, M. Kasper et al., “Lgr5 marks cycling, yet long-lived, hair follicle stem cells,” Nature Genetics, vol. 40, no. 11, pp. 1291–1299, 2008.
- V. Jaks, M. Kasper, and R. Toftgård, “The hair follicle-a stem cell zoo,” Experimental Cell Research, vol. 316, no. 8, pp. 1422–1428, 2010.
- Y. Liu, S. Lyle, Z. Yang, and G. Cotsarelis, “Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge,” Journal of Investigative Dermatology, vol. 121, no. 5, pp. 963–968, 2003.
- R. J. Morris, Y. Liu, L. Marles et al., “Capturing and profiling adult hair follicle stem cells,” Nature Biotechnology, vol. 22, no. 4, pp. 411–417, 2004.
- R. J. Morris and C. S. Potten, “Slowly cycling (label-retaining) epidermal cells behave like clonogenic stem cells in vitro,” Cell Proliferation, vol. 27, no. 5, pp. 279–289, 1994.
- M. Ohyama, A. Terunuma, C. L. Tock et al., “Characterization and isolation of stem cell-enriched human hair follicle bulge cells,” The Journal of Clinical Investigation, vol. 116, no. 1, pp. 249–260, 2006.
- H. Oshima, A. Rochat, C. Kedzia, K. Kobayashi, and Y. Barrandon, “Morphogenesis and renewal of hair follicles from adult multipotent stem cells,” Cell, vol. 104, no. 2, pp. 233–245, 2001.
- C. S. Trempus, R. J. Morris, C. D. Bortner et al., “Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34,” Journal of Investigative Dermatology, vol. 120, no. 4, pp. 501–511, 2003.
- T. Tumbar, G. Guasch, V. Greco et al., “Defining the epithelial stem cell niche in skin,” Science, vol. 303, no. 5656, pp. 359–363, 2004.
- Z. S. Morris and A. I. McClatchey, “The neurofibroma cell of origin: SKPs expand the playing field,” Cell Stem Cell, vol. 4, no. 5, pp. 371–372, 2009.